Core for Applied Genomics and Ecology (CAGE)
A Next Generation Sequencing Facility

NEWS:
Presentations from 2009 454 Workshop are now available.
FOR UNL RESEARCHERS: Next Generation Sequencing Workshop: 454 technology and applications, Spring 2010 registration is open - Download the Flier here
Download the Registration form.
Gut Function Initiative Website is live.
TRAINING: Next Generation Sequencing for Food Industry: An Alternative to Culture-based Microbiological Testing
Available upon request - Find out more and Request information!
Download the flyer in .pdf format.
Key Personnel:
Andrew K. Benson, Ph.D.(Director)
Jaehyoung Kim, Ph.D.
Chaomei Zhang, Ph.D.
Contact Information:
Jaehyoung Kim, Ph.D.
University of Nebraska-Lincoln, 325 FIC.
68583-0919
Email:jkim8@unl.edu
Tel: 402-472-5575
Fax: 402-472-1693
The 2010 Next Gen for Research Scientists workshop is scheduled for March 1 and 2.
Core for Applied Genomics and Ecology (CAGE) offers a wide variety of sequencing services. In 2008 CAGE added Massively Parallel Sequencing to its core services, using Roche-454 GS-FLX machine. In addition to printed microarrays services, the Massively Parallel Sequencing provides genome researchers with the added capabilities of high-capacity sequencing applications such as whole genome sequencing, large-scale sequencing of BACS, fosmids and metagenome libraries as well as deep sequencing applications such as 16S rRNA amplicons, allowing investigators to survey highly complex populations of microorganisms or to look for variants within uniform populations.
In March of 2009, Titanium sequencing platform became available at CAGE. This platform allows us to produce longer (up to 500 bp) reads and to accommodate up to 96 samples on a single run. You can learn more about our protocols and equipment by clicking on the links to the left.
CAGE continues to offer all of its DNA microarray services, including array design, fabrication, hybridization, image analysis, and primary data analysis. CAGE has extensive experience with cDNA arrays and oligonucleotide arrays and offers a variety of fluorescent labeling techniques, including Cy-dye incorporation, amino-allyl incorporation, or dendrimers.
In addition to NextGen Sequencing and DNA Microarray services, CAGE has substantial experience with a variety of genotyping methods including Amplified Fragment Length Polymorphism, Restriction Fragment Length Polymorphism, Pulsed Field Gel Electrophoresis, Variable Number Tandem Repeat Analysis, and Multi-Locus Sequence Typing.
To get more information about pricing for our services, simply send us an information request and we will get in touch with you shortly.

