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University of Nebraska–Lincoln

Core for Applied Genomics and Ecology

Sequencing News and Events

DNA Microarray
Total genomic DNA and RNA for comparative genomics and gene expression profiling respectively, will be purified by the user. A number of protocols are available for DNA or RNA preparation. Not all are equivalent, but we currently offer no recommendations. Our experience is that the most likely cause of microarray error is the quality of the sample, so it is critical that the utmost care go into the preparation of the samples. Please use well established protocols which give high-quality total DNA or RNA. For labeling genomic DNA, the core require that each purified DNA sample containing minimum of 30 ug total DNA at a concentration as a minimum 1 ug/ul. In case of RNA samples, we need each purified sample has at least 30 ug total RNA at a concentration no less than 1.5 ug/ul, and RNA samples must be brought to the Facility by the experimenter dissolved in RNAse free water and frozen on dry ice. The modular fee schedule described below presents our aim to provide flexibility in services and pricing suitable for many kinds of projects.
Note: Projects for clients not affiliated with the University of Nebraska will be assessed 30% on all facility fees.

  • Printing(for 3000 spots/slide) - $3,000/50 slides
  • Printing Oligomer Set - inquire
  • Labeling and Hybridization - $170/sample
  • Scanning Analysis - $25/slide/hour
  • Image Analysis - $25/sample

  • Protein Microarray
  • Printing - $350/set up - $100 substrate/array
  • Labeling (Protein)
  • Fluorescent - $300/ 100ug
  • Biotinylation - $100/ 100ug
  • Consultation - inquire
  • Genetic Profiling Techniques
  • 16S or 23S RNA Sequencing $100/sample
  • PFGE - $100/sample
  • RAPD - $50/sample
  • MLST - $300/sample
  • We provide complete MLST analysis. It includes DNA extraction, 6 PCR reaction, purification, sequencing and primary data analysis.
AFLP - We provide complete AFLP analysis. It includes DNA extraction, restriction of DNA, pre-amplification, selective amplification, and primary data analysis.